B-side Electron Transfer in Bacterial Photosynthetic Reaction Centers Revealed by a Few-Cycle Pulse Laser
1 Advanced Ultrafast Laser Research Center, University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585 Japan
2 JST, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075 Japan
3 Department of Electrophysics, National Chiao-Tung University, 1001 Ta Hsueh Rd., Hsinchu 300 Taiwan
4 Institute of Laser Engineering, Osaka University, 2-6 Yamada-oka, Suita, Osaka 565-0971 Japan
5 Department of Pharmacy, Ritsumeikan University, Kusatsu, Shiga 525-8577 Japan
B-side electron transfer in wide-type reaction centers from Rhodobacter sphaeroides 2.4.1 was investigated by broadband real-time vibrational spectroscopy. HB− was found to be generated in less than 50 fs after the excitation of 6.3 fs laser pulse, and its decay lifetime was determined to be ~280fs and 1.4ps, which is extremely fast compared with the one occurring in A-side. Considering the ultrafast of the generation time of HB−, BB rather than P was found to be the initial electron donor to the H. Hence, the initial charge-separated state is determined to be BB+HB− state.
© Owned by the authors, published by EDP Sciences, 2013
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