| Issue |
EPJ Web Conf.
Volume 335, 2025
EOS Annual Meeting (EOSAM 2025)
|
|
|---|---|---|
| Article Number | 04010 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting - BioPhotonics and Biosensors | |
| DOI | https://doi.org/10.1051/epjconf/202533504010 | |
| Published online | 22 September 2025 | |
https://doi.org/10.1051/epjconf/202533504010
Nanosecond-Resolution Integrated Microscope for High-Throughput Liquid Biopsy
1 Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy
2 Istituto di Fotonica e Nanotecnologie, IFN-CNR, Piazza Leonardo da Vinci 32, 20133 Milano, Italy
3 Istituto di Calcolo e Reti ad Alte Prestazioni, ICAR-CNR, Via P. Castellino 111, 80131 Napoli, Italy
4 Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Via Venezian 1, 20133 Milano, Italy
* e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Published online: 22 September 2025
Abstract
This work presents an integrated, high-throughput microscope on a chip designed for rapid and automated circulating tumor cells imaging based on cytomorphological features. The system employs a modified time-stretch imaging technique, utilizing a single nanosecond laser pulse split into a sequence of temporally and spatially separated pulses to illuminate the whole cell at different moments. Fabricated using femtosecond laser micromachining, the device integrates optical circuits, delay lines, and a microfluidic chip, enabling high-speed image acquisition with a single-pixel detector. The system is validated using calibration beads and tumor cells, demonstrating high resolution and stability. Fully compatible with machine learning algorithms, this platform represents a scalable, cost-effective solution for advancing real-time liquid biopsy and cancer diagnostics.
© The Authors, published by EDP Sciences, 2025
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