| Issue |
EPJ Web Conf.
Volume 335, 2025
EOS Annual Meeting (EOSAM 2025)
|
|
|---|---|---|
| Article Number | 04011 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting - BioPhotonics and Biosensors | |
| DOI | https://doi.org/10.1051/epjconf/202533504011 | |
| Published online | 22 September 2025 | |
https://doi.org/10.1051/epjconf/202533504011
Optical component integration in a microscope slide: A novel approach for super-resolution imaging
1 Dipartimento di Fisica, Politecnico di Milano, piazza Leonardo da Vinci 32, 20133, Milano, Italy
2 Istituto di Fotonica e Nanotecnologie, IFN-CNR, piazza Leonardo da Vinci 32, 20133, Milano, Italy
* Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.
Published online: 22 September 2025
Abstract
Structured illumination microscopy (SIM) is a powerful super-resolution technique that relies on patterned light to surpass the diffraction limit. However, conventional SIM setups require complex optical configurations that limit their adaptability and usability. In this work, we present an integrated optical chip fabricated using femtosecond laser micromachining, designed to simplify the generation of structured light. This chip, coupled with an optical fiber, enables direct generation of structured illumination on its surface, where the biological samples can be positioned for imaging. Our approach eliminates bulky optics, enhances system stability, and offers a compact and scalable solution for super-resolution microscopy. We present the design, the fabrication process, and the first validations of this system for advanced biological imaging.
© The Authors, published by EDP Sciences, 2025
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