| Issue |
EPJ Web Conf.
Volume 335, 2025
EOS Annual Meeting (EOSAM 2025)
|
|
|---|---|---|
| Article Number | 04013 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting - BioPhotonics and Biosensors | |
| DOI | https://doi.org/10.1051/epjconf/202533504013 | |
| Published online | 22 September 2025 | |
https://doi.org/10.1051/epjconf/202533504013
OptoRheo: Live imaging of 3D cell cultures combined with local biomechanical sensing
1 Optics and Photonics Research Group, Faculty f Engineering, University of Nottingham, Nottingham, UK.
2 Nottingham Biodiscovery Institute, School of Medicine, University of Nottingham, Nottingham, UK.
3 Institute of Biological Chemistry, Biophysics and Bioengineering, School of Engineering and Physical Sciences, Heriot Watt University, Edinburgh, UK.
4 Division of Biomedical Engineering, JamesWatt School of Engineering, University of Glasgow, Glasgow, UK.
5 School of Pharmacy, University of Nottingham, Nottingham, UK.
6 School of Veterinary Medicine & Science, University of Nottingham, Sutton Bonington Campus, Leicestershire, UK.
7 Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Published online: 22 September 2025
Abstract
OptoRheo is a new microscopy platform that allows for live imaging of cells in 3D cultures over long-time courses, combined with micromechanical sensing of the material local to the cells. This is achieved by combining light sheet microscopy, multiplane imaging, optical trapping, and passive particle tracking micro-rheology in a single optical platform. A novel light sheet configuration allows cells to remain undisturbed during imaging, with no dipping objectives or sample scanning involved, allowing delicate samples to grow on the microscope stage over several days. This talk will demonstrate the capabilities of OptoRheo by studying two different cell culture systems, cell cultures grown in hydrogel and spheroid samples.
© The Authors, published by EDP Sciences, 2025
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