| Issue |
EPJ Web Conf.
Volume 363, 2026
International Conference on Low-Carbon Development and Materials for Solar Energy (ICLDMS’26)
|
|
|---|---|---|
| Article Number | 03004 | |
| Number of page(s) | 13 | |
| Section | Computational and Biological Materials | |
| DOI | https://doi.org/10.1051/epjconf/202636303004 | |
| Published online | 16 April 2026 | |
https://doi.org/10.1051/epjconf/202636303004
Microbial Alkaline Proteases as a Greener Aid to Eco-Sustainable Detergent: Actions to Addition
1 Department of Biotechnology, Sathyabama Institute of Science and Technology, Dr. Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India.
2 Centre for Molecular and Nanomedical Sciences, Sathyabama Institute of Science and Technology, Dr. Jeppiaar Nagar, Rajiv Gandhi Salai, Chennai - 600 119, Tamil Nadu, India
* Corresponding Author: Name: Dayanandan Anandan Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Published online: 16 April 2026
Abstract
Alkaline proteases are among the most important industrial enzymes, widely used in detergent formulations due to their high efficiency, specificity, and eco-friendly nature. These enzymes break down proteinbased stains such as blood, milk, eggs, and sweat by converting insoluble proteins into soluble peptides and amino acids under alkaline conditions. This enhances cleaning performance while maintaining fabric quality. Although proteases are produced by plants, animals, and microorganisms, microbial sources are preferred because of their high yield, stability, and ease of genetic modification. Industrially important microorganisms such as Bacillus, Aspergillus, and Trichoderma produce proteases that are more stable and cost-effective compared to plant- and animal-derived enzymes like papain and trypsin. Microbial alkaline proteases play a key role in laundry detergents, dishwashing agents, and industrial cleaners, allowing effective stain removal even at lower temperatures and reducing the need for harsh chemicals. Advances in protein engineering, recombinant DNA technology, and directed evolution are further improving enzyme stability and performance. Additionally, the use of agro-industrial waste for enzyme production offers a sustainable and economical approach. Overall, alkaline proteases provide an efficient and environmentally friendly solution, supporting greener cleaning technologies and sustainable industrial development.
Key words: Alkaline protease / eco-sustainable detergent / greener aid
© The Authors, published by EDP Sciences, 2026
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