Issue |
EPJ Web Conf.
Volume 287, 2023
EOS Annual Meeting (EOSAM 2023)
|
|
---|---|---|
Article Number | 03018 | |
Number of page(s) | 2 | |
Section | Topical Meeting (TOM) 3- Biophotonics | |
DOI | https://doi.org/10.1051/epjconf/202328703018 | |
Published online | 18 October 2023 |
https://doi.org/10.1051/epjconf/202328703018
Broadband CARS high-throughput single-cell imaging
1 Department of Electronic Engineering, Maynooth University, Co. Kildare, Ireland
2 Department of Computer Science, Maynooth University, Maynooth, Co. Kildare, Ireland
* e-mail: ryan.muddiman.2021@mumail.ie
** e-mail: bryan.hennelly@mu.ie
Published online: 18 October 2023
Broadband Coherent anti-Stokes Raman Scattering (BCARS) enables the whole vibrational spectrum of cytologically prepared samples to be obtained using a hyperspectral raster scan approach. This technique has the potential to enable high-throughput automated detection of cell abnormalities. Images are distorted by the non-resonant background which requires a treatment for proper analysis. Using statistical denoising and phase retrieval returns Raman spectra similar to that of a spontaneous Raman measurement. Here, we present our work using this method for single-cell imaging of PEO1 ovarian adenocarcinoma cells prepared with the ThinPrep processor which enables label-free Raman cytology.
© The Authors, published by EDP Sciences, 2023
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