| Issue |
EPJ Web Conf.
Volume 335, 2025
EOS Annual Meeting (EOSAM 2025)
|
|
|---|---|---|
| Article Number | 04004 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting - BioPhotonics and Biosensors | |
| DOI | https://doi.org/10.1051/epjconf/202533504004 | |
| Published online | 22 September 2025 | |
https://doi.org/10.1051/epjconf/202533504004
Generation and Characterization of Vector Beams with a Single SLM for Super-Resolution Microscopy
1 Department de Física Aplicada, Facultat de Física, Universitat de Barcelona, Carrer de Martí i Franquès 1, 08028 Barcelona, Spain
2 Institut de Nanociència i Nanotecnologia (IN2UB), 08028, Barcelona, Spain
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Published online: 22 September 2025
Abstract
We present a method to generate vector beams using a single spatial light modulator (SLM) for depletion-based super-resolution microscopy techniques such as STED, RESOLFT, and subtraction microscopy. We demonstrate the generation of circularly polarized Laguerre-Gaussian LG01 beams and vector beams with spatially varying polarization, such as azimuthal beams. Their intensity profiles, polarization distributions, and robustness to optical aberrations are experimentally analyzed under low-and high-numerical aperture conditions to assess their suitability for super-resolution applications.
© The Authors, published by EDP Sciences, 2025
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