| Issue |
EPJ Web of Conferences
Volume 41, 2013
XVIIIth International Conference on Ultrafast Phenomena
|
|
|---|---|---|
| Article Number | 11009 | |
| Number of page(s) | 3 | |
| Section | Frequency Combs, Waveform Synthesis, Pulse Shaping and Applications | |
| DOI | https://doi.org/10.1051/epjconf/20134111009 | |
| Published online | 13 March 2013 | |
https://doi.org/10.1051/epjconf/20134111009
Pulse-shaping-based two-photon FRET microscopy
1 Applied Physics Program, University of Michigan, 450 Church Street, Ann Arbor, MI, USA 48109
2 Department of Physics and Biophysics, University of Michigan, 450 Church Street, Ann Arbor, MI, USA 48109
3 Department of Microbiology and Immunology, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI, USA 48109
We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.
© Owned by the authors, published by EDP Sciences, 2013
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