Issue |
EPJ Web of Conferences
Volume 77, 2014
16th International Congress of Metrology
|
|
---|---|---|
Article Number | 00007 | |
Number of page(s) | 4 | |
DOI | https://doi.org/10.1051/epjconf/20147700007 | |
Published online | 19 August 2014 |
https://doi.org/10.1051/epjconf/20147700007
Metrological Approach for the Quantification of Metalloproteins (EMRP Project HLT05)
1 Physikalisch-Technische Bundesanstalt, Bundesallee 100, 38108 Braunschweig, Germany
2 Laboratoire National de Métrologie et d’Essais, 1, rue Gaston Boissier, 75724 Paris Cedex 15, France
3 LGC, Queens Road, Teddington, Middlesex, TW11 0LY, UK
a Corresponding author: claudia.swart@ptb.de
Published online: 19 August 2014
About 30% of all proteins contain metals either as cofactor or covalently bound as part of the protein. Some of these metalloproteins such as transferrin (TRF) and haemoglobin (HGB) are regularly measured in clinical laboratories. However, for most of them, no primary reference measurement procedures exist, although directives such as the EC-directive covering in vitro diagnostic medical devices ((IVD-) directive 98/79/EC) and standards such as EN ISO 17511:2003 demand the traceability of results received for analytes in samples of human origin. The EMRP project HLT05 aims at establishing a traceability chain for metalloproteins as is already in place for some inorganic and small organic molecules in biological samples.
Résumé
Environ 30 % de toutes les protéines contiennent des métaux, soit en tant que cofacteur, soit faisant partie de la protéine par liaison covalente. Certaines de ces métalloprotéines, telles que la transferrine (TRF) et l'hémoglobine (HGB), sont régulièrement analysées en laboratoires cliniques. Cependant, pour la plupart d'entre elles, il n'existe pas de méthode de mesure de référence primaire bien que des directives, telles que la directive CE relative aux dispositifs médicaux de diagnostic in vitro (directive (IVD) 98/79/CE), et des normes, telles que l'EN ISO 17511:2003, exigent la traçabilité des résultats pour les analytes dans les échantillons d'origine humaine. Le projet HLT05 de l'EMRP a pour objectif de mettre en place pour les métalloprotéines une chaîne de traçabilité similaire à celle existant déjà pour les molécules inorganiques et les petites molécules organiques dans les échantillons biologiques.
© Owned by the authors, published by EDP Sciences, 2014
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