EPJ Web Conf.
Volume 190, 2018XIII International Conference on Hole Burning, Single Molecule, and Related Spectroscopies: Science and Applications (HBSM-2018)
|Number of page(s)||2|
|Published online||26 September 2018|
Thin layer fluorescence microscopy based on one-dimensional photonic crystal
Federal State Budgetary Institution Federal Research and Clinical Center Of Physical-Chemical Medicine, Federal Medical Biological Agency, Laboratory of Medical Nanotechnologies, 119435 Malaya Pirogovskaya 1a, Moscow, Russia
2 Moscow institute of physics and technology, Departure of Biological and Medical Physics, 141701 Institutskiy per. 9, Dolgoprudny, Moscow Region, Russia
* Corresponding author: firstname.lastname@example.org
Published online: 26 September 2018
A new method of specimen illumination for wide-field fluorescence microscopy has been presented. This method allows to excite the fluorescence in a thin near-surface layer of the studied object. As a result, the captured images have greater contrast and signal-to-background ratio in comparison with the epifluorescence ones. The long-range surface waves in one-dimensional photonic crystal have been used to localize the electromagnetic field exciting the fluorescence. An experimental setup has been created to excite the surface waves and obtain images of the objects from the near-surface layer. For an illustration of the possibilities of our method, we conducted several experiments with specimens that are typical for fluorescence microscopy, such as bacteria and eukaryotic cells.
© The Authors, published by EDP Sciences, 2018
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