| Issue |
EPJ Web Conf.
Volume 255, 2021
EOS Annual Meeting (EOSAM 2021)
|
|
|---|---|---|
| Article Number | 11009 | |
| Number of page(s) | 4 | |
| Section | Topical Meeting (TOM) 13- Ultrafast Optical Technologies and Applications | |
| DOI | https://doi.org/10.1051/epjconf/202125511009 | |
| Published online | 18 November 2021 | |
https://doi.org/10.1051/epjconf/202125511009
A Spectral Resolution study in Femtosecond Stimulated Raman Scattering Microscopy
1 National Research Council (CNR), Institute of Applied Sciences and Intelligent Systems, Napoli, Italy
2 CHT @Erzelli, Nanoscopy Istituto Italiano Di Tecnologia, Genova, Italy
* Corresponding author: rajeev.ranjan@iit.it
Published online: 18 November 2021
The simultaneous mapping and the specificity of different chemical species are desirable in several biological and biomedical applications. The stimulated Raman Scattering technique is a proven and well-established label-free method to map the distributions of individual species in a multi-component-based system due to the linear dependence of signals on concentration and its chemical selectivity. In this framework, spectral resolution, i.e., the ability to distinguish closely lying resonances, plays a fundamental role. Here in this work, the cross-correlation of Ti:Sa & OPO femtosecond laser beams in a stimulated Raman scattering microscope is measured. The separation between protein and lipid bands in the C-H region is important for biochemical research and is successfully classified.
© The Authors, published by EDP Sciences, 2021
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