| Issue |
EPJ Web Conf.
Volume 266, 2022
EOS Annual Meeting (EOSAM 2022)
|
|
|---|---|---|
| Article Number | 12007 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 12- Optofluidics | |
| DOI | https://doi.org/10.1051/epjconf/202226612007 | |
| Published online | 13 October 2022 | |
https://doi.org/10.1051/epjconf/202226612007
Size-based chromosome separation in a microfluidic particle separation device using viscoelastic fluids
1 Department of Health Technology, Technical University of Denmark, Ørsteds Plads, Building 345C, 2800 Kgs. Lyngby, Denmark
2 Center for Chromosome Stability, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 København N., Denmark
* Corresponding author: akri@dtu.dk
Published online: 13 October 2022
Viscoelastic flow-based particle manipulation techniques enable bio-particle focusing, separation, and enrichment by precisely tuning the rheological parameters, flow conditions, and microchannel geometry. In this study, we fabricated a PDMS-based single inlet/outlet microchannel to separate bio-particles by their size ranging from 1-10 μm. Flow conditions and rheological properties are optimized using 2 μm and 4 μm Polystyrene beads to reach the best particle separation condition. We demonstrated the size-based separation of human chromosomes by separating 1-2 μm size small chromosomes from 8-10 μm size large chromosomes. Thanks to its miniaturized size and simplicity, the isolation chip and unique viscoelastic separation method have great potential to be used as a future pioneering tool for genetic applications to study chromosome abnormalities such as fragile-X and trisomy.
© The Authors, published by EDP Sciences
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