| Issue |
EPJ Web Conf.
Volume 309, 2024
EOS Annual Meeting (EOSAM 2024)
|
|
|---|---|---|
| Article Number | 04006 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 4- BioPhotonics and Biosensors | |
| DOI | https://doi.org/10.1051/epjconf/202430904006 | |
| Published online | 31 October 2024 | |
https://doi.org/10.1051/epjconf/202430904006
Development of a Microfluidic Device for Blood Cells Extraction in Liquid Biopsy
1 Dipartimento Interateneo di Fisica Michelangelo Merlin, University & Polytechnic of Bari, 70126 Via Giovanni Amendola 173, Italy
2 Universitat Politecnica de Catalunya (UPC), 08222 Campus de Terrassa Carrer de Colom 1, Spain
3 CNR-IFN UOS BARI, Via Amendola 173, 70125 Bari, Italy
4 CNR-IFN UOS Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy
* Corresponding author: caragnanostefania@gmail.com
Published online: 31 October 2024
This project aims to produce a microfluidic device capable of separating 6 μm and 20 μm diameters particles by inertial sorting. This Lab-on-Chip (LoC) was designed with a trapezoidal cross-section for better fluid control and effective particle manipulation at the microscopic level, as demonstrated by COMSOL simulations. The device was manufactured on a substrate of Polymethyl Methacrylate (PMMA) by femtosecond laser technology and then assembled using an innovative geometry-preserving Isopropyl alcohol-based procedure. The LoC was test with spherical plastic microparticles of two diameters (6 μm and 20 μm) suspended in distilled water. The separation efficiencies were (98.2 ± 1.6) % for 20 μm diameter particles and (70.0 ± 1.8) % for 6 μm diameter particles in good agreement with the simulation results. Finally, after a microfluidic channels’ acetone vapors treatment, the device demonstrated a good ability to separate biological particles (Red Blood Cells) at different concentrations (20%, 30%, 40%, 50%) in a PBS buffer.
© The Authors, published by EDP Sciences, 2024
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