| Issue |
EPJ Web Conf.
Volume 309, 2024
EOS Annual Meeting (EOSAM 2024)
|
|
|---|---|---|
| Article Number | 10026 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 10- Applications of Optics and Photonics | |
| DOI | https://doi.org/10.1051/epjconf/202430910026 | |
| Published online | 31 October 2024 | |
https://doi.org/10.1051/epjconf/202430910026
Precision localization of cellular proteins with fluorescent Fab-based probes
1. Istituto di Endocrinologia ed Oncologia Sperimentale, Consiglio Nazionale delle Ricerche, Napoli, Italia
2. Cardiovascular Research Institute, University of California San Francisco, San Francisco, USA
3. Department of Biotechnology and Molecular Medicine (MMBM) School of Medicine, University Federico II, Napoli, Italia
4. Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany
5. Istituto di Scienze Applicate e Sistemi Intelligenti, Consiglio Nazionale delle Ricerche, Napoli, Italia
Published online: 31 October 2024
With continuously improving resolution of today’s (super-resolution) microscopes, a major technical limitation of light microscopy based image analysis is linkage error – a visualization error that is measured by the distance between the cellular target to be detected and the fluorescence emitter used for detection. The linkage error of standard labelled antibodies is caused by the size of the antibody and the random distribution of fluorescent emitters on the antibody surface. In this study, we describe a class of staining reagents that effectively reduce the linkage error by more than five-fold when compared to conventional staining techniques. We believe this class of reagents realize an unmet need in cell biological super resolution imaging studies where the precise localization of the target of interest is crucial for the understanding of complex biological phenomena
© The Authors, published by EDP Sciences, 2024
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