| Issue |
EPJ Web Conf.
Volume 309, 2024
EOS Annual Meeting (EOSAM 2024)
|
|
|---|---|---|
| Article Number | 14003 | |
| Number of page(s) | 2 | |
| Section | Focused Sessions (FS) 4- Photonics for Cultural Heritage | |
| DOI | https://doi.org/10.1051/epjconf/202430914003 | |
| Published online | 31 October 2024 | |
https://doi.org/10.1051/epjconf/202430914003
Characterization of degradation effects on wood ultrastructure by non-linear imaging
1 Consiglio Nazionale delle Ricerche - Istituto Nazionale di Ottica, Largo e. Fermi 6, 50125, Firenze
2 Università degli Studi di Firenze - Dipartimento di Scienze e Tecnologie Agrarie, Alimentari, Ambientali e Forestali (DAGRI), Piazzale delle Cascine, 18 - 50144 Firenze (FI)
* Corresponding author: alice.dalfovo@ino.cnr.it
Published online: 31 October 2024
The characterization of deterioration processes in wooden artifacts is crucial for assessing their state of conservation and ensuring their preservation. Advanced imaging techniques are currently being explored to study the effect of chemical changes on the structural and mechanical properties of wood. Combined second harmonic generation and two-photon excited fluorescence (SHG/TPEF) imaging is a recently introduced non-destructive method for the analysis of cellulose-based samples. The study of age-related wood degradation based on nonlinear signal variation is a promising avenue. This work involves nonlinear multimodal analysis of naturally aged and dendrochronologically dated spruce samples. SHG/TPEF imaging and fluorescence lifetime imaging microscopy (FLIM) were used to demonstrate the influence of molecular deterioration and rearrangement of biopolymers on the fluorescence emitted by lignin and the second harmonic signal generated by cellulose. Imaging based on spectral filter detection and time-resolved analysis of the nonlinear fluorescence signal was used to delineate and potentially quantify ageing-induced morpho-chemical changes in the ultrastructure of wood cells. The analysis of cell structures by optical sectioning revealed variations between wood samples of different ages and different cell structures.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.