EPJ Web of Conferences
Volume 83, 2015QENS/WINS 2014 - 11th International Conference on Quasielastic Neutron Scattering and 6th International Workshop on Inelastic Neutron Spectrometers
|Number of page(s)||4|
|Published online||23 January 2015|
Vibrational dynamics of plant light-harvesting complex LHC II investigated by quasi- and inelastic neutron scattering
1 Tartu University, Institute of Physics, Tartu, Estonia
2 Department of Life Science & Technology, Laboratory of Biochemistry, University for Applied Sciences, Berlin, Germany
3 Institute of Physical Energetics, Riga, Latvia
a Corresponding author: firstname.lastname@example.org
Published online: 23 January 2015
Vibrational dynamics of the light-harvesting complex II (LHC II) from spinach was investigated by quasi- and inelastic neutron scattering (QENS and INS) at three different temperatures of 80, 160, and 285 K. QENS/INS spectra of solubilised LHC II and of the corresponding buffer solution were obtained separately and exhibit characteristic inelastic features. After subtraction of the buffer contribution, the INS spectrum of LHC II reveals a distinct Boson peak at ∼ 2.5 meV at 80 K that shifts towards lower energies if the temperature is increased to 285 K. This effect is interpreted in terms of a “softening” of the protein matrix along with the dynamical transition at ∼ 240 K. Our findings indicate that INS is a valuable method to obtain the density of vibrational states not only at cryogenic, but also at physiological temperatures.
© Owned by the authors, published by EDP Sciences, 2015
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