EPJ Web Conf.
Volume 185, 2018Moscow International Symposium on Magnetism (MISM 2017)
|Number of page(s)||4|
|Section||Magnetism in Biology and Medicine|
|Published online||04 July 2018|
Selected features of morpho-functional reactions of eukaryotic microorganisms grown in the presence of maghemite iron oxide nanoparticles obtained by laser target evaporation
FSBEI HE Irkutsk State Medical University MOH, Red Revolt str., 1, Irkutsk, 664003, Russia
2 Irkutsk State University, Nizhnyaya Embankment St., 6, Irkutsk, 664011, Russia
3 Ural Federal University, Mira St. 19 Ekaterinburg, 620000, Russia
4 Institute of Electrophysics, Amundsen str., 106, Ekaterinburg, 620016, Russia
5 Universidad del País Vasco UPV-EHU, Leioa, 48940 Spain
Corresponding author : firstname.lastname@example.org
Published online: 4 July 2018
Biological activity of iron introduced into nutrient medium as a suspension of iron oxide nanoparticles (MNPs) synthesized by the laser target evaporation was investigated. Exophiala nigrum (E. nigrum) eukaryotes were grown either in the presence or in absence of MNPs. De-aggregated suspensions of Fe2.75O4 MNPs were added in concentrations of 1 to 104 maximum permissive dose (MPD, being 0.3 mg/L of Fe ions in water). Cells were exposed for 24 to 96 hours periods and then plated onto a solid medium. The effect of MNPs was evaluated by the change in the number of cells during exposure and the number and morphology of the colonies. For 1-10 MPD yeast showed unaltered characteristics. For 100 or 1000 MPD for 72 hours of exposure and above the number of cells increases up to 30 times in comparison with the control. A pronounced stimulating effect was revealed at 104 MPD of iron. A significant excess of the number of cells was observed for the first day. At exposures of 72 and 96 hours the differences in the number of cells in comparison with the control were 11 and 30 times, respectively. A change in the colonies morphology was observed at 100 MPD concentration.
© The Authors, published by EDP Sciences, 2018
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