Methodological aspects of small iron concentrations determination in black yeasts grown in the presence of iron oxide nanoparticles

¹ Ural Federal University, Mira str., 19, Ekaterinburg, 620002, Russia
² Irkutsk State University, Nizhnyaya Embankment St., 6, Irkutsk, 664011, Russia
³ Institute of Electrophysics, Amundsen str., 106, Ekaterinburg, 620016, Russia
⁴ Universidad del País Vasco UPV-EHU, SGIKER, Leioa, 48940, Spain
⁵ Universidad del País Vasco UPV-EHU, Depto Electricidad y Electrónica, Leioa, 48940, Spain

^* Corresponding author: grisha2207@list.ru

Published online: 4 July 2018

Abstract

Nonpathogenic Exophiala nigrum (black yeasts) unicelular organisms of the Baikal Lake were used as a model system for determination of small iron concentrations in the samples grown without or with controlled amount of maghemite nanoparticles (MNPs) in nutrient. MNPs were produced by the electrophysical laser target evaporation technique. Electrostatically stabilized suspensions were prepared using sodium citrate solutions in distilled water. We assumed that one maximum permissive dose of ionic iron in water 1 MPD is equal to 0.3 mg/L. For biological experiments Saburo liquid nutrient medium was prepared with iron concentrations of 0, 10², 10³ and 10⁴ MPD. One ml of E. Nigrum cell suspension was added to Saburo liquid nutrient for 24 hours exposure. Followed by sowing onto a solid agar Saburo for 30 days colonies grows. Biosamples for electron microscopy, magnetic and total reflection X-ray fluorescence spectroscopy measurements were collected simultaneously. We were able to comparatively analyze the trace concentrations of iron in the yeast of the order of 10 ppm for control group and 600 ppm for the group grown in the presence of 10⁴ MPD of iron.