| Issue |
EPJ Web Conf.
Volume 266, 2022
EOS Annual Meeting (EOSAM 2022)
|
|
|---|---|---|
| Article Number | 13014 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 13- Advances and Applications of Optics and Photonics | |
| DOI | https://doi.org/10.1051/epjconf/202226613014 | |
| Published online | 13 October 2022 | |
https://doi.org/10.1051/epjconf/202226613014
Advancing fluorescence microscopy and spectroscopy for the benefit of cell science and drug delivery
1 Photonics4Life research group, Departmento de Física Aplicada, Facultade de Física and Instituto de Materiais (iMATUS), Universidade de Santiago de Compostela, Campus Vida, E-15782 Santiago de Compostela, Spain
2 International Iberian Nanotechnology Laboratory, Avenida Mestre José Veiga s/n, Braga, Portugal
3 JPK BioAFM, Bruker Nano GmbH, Am Studio 2D, 12489 Berlin, Germany
* Corresponding author: anaisabel.gomez@usc.es
Published online: 13 October 2022
Here we report on two recent advances we made applying advanced fluorescence microscopy and spectroscopy to further the study of cell biology and drug delivery. At first, we detail a new instrumental set-up combining atomic force microscopy in liquid and super-resolution fluorescence microscopy in novel configuration such that long-term simultaneous and co-localized observation with both techniques becomes feasible. We believe this will contribute to the study of fast membrane activated cell-signalling processes in the years to come. Furthermore, we report on a novel application of fluorescence cross-correlation spectroscopy (FCCS) for the characterization of lipid-nucleic acid complexes. We could determine the number of nucleic acid particles incorporated in each liposome, a parameter not readily accessible on ensemble basis with other methods. This parameter is crucial for co-delivery applications, and we believe FCCS can play an important role in the future development of new drug delivery systems.
© The Authors, published by EDP Sciences
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