| Issue |
EPJ Web Conf.
Volume 287, 2023
EOS Annual Meeting (EOSAM 2023)
|
|
|---|---|---|
| Article Number | 02004 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 2- Adaptive and Freeform Optics | |
| DOI | https://doi.org/10.1051/epjconf/202328702004 | |
| Published online | 18 October 2023 | |
https://doi.org/10.1051/epjconf/202328702004
Modulating phase for adaptive optics and PSF shaping in bio-imaging: Requirements & development of a new deformable mirror tailored to microscopy
Imagine Optic, 91400 Orsay, France
* Corresponding author: fharms@imagine-optic.com
Published online: 18 October 2023
Modern bio-imaging techniques such as light-sheet, multiphoton and PALM/STORM are now aiming to image more complex biological samples at larger depth and therefore face larger-amplitude and more complex aberrations. We provide an analysis of key requirements driving optimal implementation of adaptive optics (AO) in microscopy, with a focus on wavefront modulators. We show that some specifications of wavefront modulators such as linearity, hysteresis or actuators performance & layout can end up to better AO performance in microscopy systems, when specifically optimized for such use. We then provide design details and characterization results of a newly developed deformable mirror, and report on experimental images obtained from AO-enhanced microscopes based on the device, for several modalities such as light-sheet, multiphoton or super-resolution single molecule localization systems. Finally, we provide recommendations on how to define the right set of AO components, algorithms and overall method depending on modality, instrument and sample constraints.
© The Authors, published by EDP Sciences, 2023
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