| Issue |
EPJ Web Conf.
Volume 309, 2024
EOS Annual Meeting (EOSAM 2024)
|
|
|---|---|---|
| Article Number | 04011 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 4- BioPhotonics and Biosensors | |
| DOI | https://doi.org/10.1051/epjconf/202430904011 | |
| Published online | 31 October 2024 | |
https://doi.org/10.1051/epjconf/202430904011
Implantable Micro-optics for label-free non-linear imaging
1 Department of Physics, Università di Milano-Bicocca, Piazza della Scienza 3, 20126, Milan, Italy
2 Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, Piazza L. da Vinci 32, 20133 Milan, Italy
3 Institute for Photonics and Nanotechnologies (IFN)-, CNR and Department of Physics, Politecnico di Milano, Piazza L. da Vinci 32, 20133 Milan, Italy
4 Division of Immunology, Transplantation and Infectious Diseases IRCCS San Raffaele Scientific Institute, Vita-Salute San Raffaele University, Milan, Italy
5 FORTH/IESL Plastira 100, 70013 Heraklion, Greece
6 Institute of Nanoscience and Nanotechnology, NCSR Demokritos, Athens, Greece
* Corresponding author: giuseppe.chirico@unimib.it
Published online: 31 October 2024
Non-linear excitation microscopy offers superior in-vivo imaging but faces challenges in deep tissue. High numerical aperture beams suffer spherical aberrations, while tissue scattering impacts image quality. To address this, we propose implantable microlenses for precise focusing below the skin in lab animals. By using low numerical aperture lasers, we avoid spherical aberrations induced by high NA objectives. Our study presents various microlens designs differing in size, shape, and fabrication methods, all on glass or organo-hybrid ceramic substrates. This approach shows promise for enhancing deep tissue imaging, facilitating better understanding of biological processes in vivo.
© The Authors, published by EDP Sciences, 2024
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