| Issue |
EPJ Web Conf.
Volume 287, 2023
EOS Annual Meeting (EOSAM 2023)
|
|
|---|---|---|
| Article Number | 03009 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 3- Biophotonics | |
| DOI | https://doi.org/10.1051/epjconf/202328703009 | |
| Published online | 18 October 2023 | |
https://doi.org/10.1051/epjconf/202328703009
Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy
1 Department of Physics, Politecnico di Milano, Milan, Italy
2 Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Milan, Italy
3 Department of Physics, Università degli Studi di Milano-Bicocca, Milan, Italy
4 Institute for Photonics and Nanotechnologies (IFN), CNR, Milan, Italy
* Corresponding author: behjatsadat.kariman@polimi.it
Published online: 18 October 2023
A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens.
© The Authors, published by EDP Sciences, 2023
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