| Issue |
EPJ Web Conf.
Volume 287, 2023
EOS Annual Meeting (EOSAM 2023)
|
|
|---|---|---|
| Article Number | 09015 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 9- Applications in Optics and Photonics | |
| DOI | https://doi.org/10.1051/epjconf/202328709015 | |
| Published online | 18 October 2023 | |
https://doi.org/10.1051/epjconf/202328709015
Microstructured molecular BIO-gratings by means of UV induced denaturation - INVITED
1 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46022 Valencia, Spain
2 Laboratory of Fiber Optics - Institut de Ciència dels Materials (ICMUV), Universitat de València, Catedrático José Beltrán 2, 46980 Paterna,Valencia, Spain
3 Departament de Química, Universitat Politècnica de València, 46022 Valencia, Spain.
* Corresponding author: martina.delgado@uv.es
Published online: 18 October 2023
Rapid, reliable and low cost techniques to fabricate biosensors is a hot topic nowadays. Here, we present a BIO-grating fabricated by means of local, selective denaturing of molecules using UV radiation. A phase-mask is used to generate an interferometric pattern of 1420 nm pitch that, when illuminating a biolayer of BSA molecules lead to its periodic deactivation. After the biorecognition of the specific antibody, aBSA, a BIO-grating is generated due to the height difference between the protein, and the complex protein + antibody. We present the optimization of the fabrication of the BIO-gratings and their AFM characterization. Also, the biosensor performance in terms of limit of detection and limit of quantification will be presented.
© The Authors, published by EDP Sciences, 2023
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