| Issue |
EPJ Web Conf.
Volume 287, 2023
EOS Annual Meeting (EOSAM 2023)
|
|
|---|---|---|
| Article Number | 03011 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 3- Biophotonics | |
| DOI | https://doi.org/10.1051/epjconf/202328703011 | |
| Published online | 18 October 2023 | |
https://doi.org/10.1051/epjconf/202328703011
Volumetric one-photon UVA hyperspectral light sheet imaging in mouse pre-implantation embryos - INVITED
1 SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, United Kingdom
2 Robinson Research Institute, School of Biomedicine, The University of Adelaide, Adelaide, Australia
3 Centre of Light for Life and School of Biological Sciences, The University of Adelaide, Adelaide, 5005, Australia
* Corresponding author: jmm46@st-andrews.ac.uk
Published online: 18 October 2023
UVA one-photon hyperspectral light sheet imaging was performed here to capture and reconstruct three dimensional metabolic maps of mouse embryos during development. We explored numerically and experimentally the advantages of phasor-based hyperspectral approach for detecting embryo metabolism with a single wavelength excitation compared to the conventional detection approach using bandpass filters.
© The Authors, published by EDP Sciences, 2023
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