| Issue |
EPJ Web Conf.
Volume 287, 2023
EOS Annual Meeting (EOSAM 2023)
|
|
|---|---|---|
| Article Number | 03012 | |
| Number of page(s) | 2 | |
| Section | Topical Meeting (TOM) 3- Biophotonics | |
| DOI | https://doi.org/10.1051/epjconf/202328703012 | |
| Published online | 18 October 2023 | |
https://doi.org/10.1051/epjconf/202328703012
Imaging of calcium gradient oscillations in plant root hairs by light sheet fluorescence microscopy
1 Politecnico di Milano, Dipartimento di Fisica, 20133 Milano, Italy
2 Università degli Studi di Milano, Dipartimento di Bioscienze, 20133 Milan, Italy
* Corresponding author: andrea1.bassi@polimi.it
Published online: 18 October 2023
Root hairs are a delicate single-cell system whose growth is regulated by a fine mechanism characterised by the presence of a tip-high Ca2+ gradient that shows regular oscillations in growing root hairs. We show a method based on the use of Light sheet fluorescence microscopy (LSFM) which allows the quasi-physiological analysis of Arabidopsis thaliana plant roots hairs with excellent spatial and temporal resolution over a wide field of view. We show how the healthy growing root hairs are linked to precise oscillations and how a disruption of this mechanism can be associated to specific genes.
© The Authors, published by EDP Sciences, 2023
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